TOP2A (17q21) / SE 17
The Topoisomerase2A enzyme, which is vital for the cell because of its role in cell replication and repair, catalyzes the relaxation of supercoiled DNA molecules to create a reversible double-strand DNA break.
This enzyme is also the target of a number of cytotoxic agents, namely TOP2A inhibitors (anthracyclines, etoposide, teniposide).
The presence of both TOP2A amplification and deletion in advanced cancer are associated with decreased survival, and occur frequently and concurrently with Her2 gene amplification.
The Dual-Color Probe is optimized to detect amplifications (copy-numbers) or deletions of the TOP2A gene region at the 17q21. In order to expand the utility of the FISH assay, the TOP2A (17q21) / Her2/neu (17q12) / SE17 probe is designed as a triple-color assay to detect amplification at 17q12 and amplifications or deletions at 17q21.
Cat.# KBI-10724 Top2A (17q21) / SE 17
TOP2A (17q21) / SE 17 probe hybridized to breast tissue (2R2G).
Literature:
Järvinen et al, 1999, Genes, Chromosomes and Cancer 26; 142-150
Järvinen et al, 2000, Am. J. Pathology 156; 639-647