Introduction
The FISHBright™ labeling kits are based on Kreatech’s Universal Linkage System (ULS™). The ULS molecule is a proprietary platinum compound linked to a hapten or a fluorophore. The FISHBright kits offer ULS molecules coupled to a variety of fluorophores or haptens providing a non-enzymatic, easy-to-use alternative to conventional techniques such as nick translation, random priming, end labeling, etc.
The FISHBright™ labeling kits are specially developed for FISH applications: labeled DNA probes can be used on all type of samples including Metaphase spreads, direct Interphase cell preparation (e.g. blood smears, bone marrow smears), urine samples, paraffin-embedded or frozen tissue sections.
Kreatech’s Poseidon probes are labeled with the same technology and all Poseidon pretreatment kits can be used in combination with DNA probes which are labeled with the FISHBright™ labeling kits.
Besides the ULS technology the kits also contain cell specific hybridization buffer (CHB), and Tissue Hybridization Buffer (THB). Also the KREAboost solution, which is developed to generate a high signal to noise ratio especially on paraffin-embedded tissue sections, is included in these kits.
The FISHBright™ labeling kits will offer you all advantages of the ULS technology:
- Simple to use
- Labeling procedure within 30 minutes
- Use of KREApure columns: a unique purification column which guarantees a very high removal of non reacted ULS (>99%) and high sample recoveries (> 95%)
Content FISHBright™ Labeling Kits
- PlatinumBright component (fluorochromes or haptens)
- 10 x Labeling Solution
- KREApure purification columns
- Hybridization buffer for cytological preps (CHB)
- Hybridization buffer for paraffin preps (THB KREAboost)
The FISHBright™ Kit contains sufficient solution to label up to 10 μg of BAC DNA, which generally allows to perform 100 or more FISH assays using 10 μl labeled probe in hybridization buffer (amount of FISH assays is dependent on the final concentration of labeled DNA. Usually 50 – 100 ng/ μl of labeled BAC DNA is necessary per assay).
FISHgrade C0t
FISHgrade C0t DNA is used to suppress cross-hybridization to human repetitive DNA in FISH experiments. FISHgrade C0t is a high quality C0t-1 DNA that effectively represses repetitive sequences in in situ hybridization experiments. It works perfectly for probes labeled with FISHBright ULS-Kits.
Introduction
The FISHBright™ labeling kits are based on Kreatech’s Universal Linkage System (ULS™). The ULS molecule is a proprietary platinum compound linked to a hapten or a fluorophore. The FISHBright kits offer ULS molecules coupled to a variety of fluorophores or haptens providing a non-enzymatic, easy-to-use alternative to conventional techniques such as nick translation, random priming, end labeling, etc.
The FISHBright™ labeling kits are specially developed for FISH applications: labeled DNA probes can be used on all type of samples including Metaphase spreads, direct Interphase cell preparation (e.g. blood smears, bone marrow smears), urine samples, paraffin-embedded or frozen tissue sections.
Kreatech’s Poseidon probes are labeled with the same technology and all Poseidon pretreatment kits can be used in combination with DNA probes which are labeled with the FISHBright™ labeling kits.
Besides the ULS technology the kits also contain cell specific hybridization buffer (CHB), and Tissue Hybridization Buffer (THB). Also the KREAboost solution, which is developed to generate a high signal to noise ratio especially on paraffin-embedded tissue sections, is included in these kits.
The FISHBright™ labeling kits will offer you all advantages of the ULS technology:
- Simple to use
- Labeling procedure within 30 minutes
- Use of KREApure columns: a unique purification column which guarantees a very high removal of non reacted ULS (>99%) and high sample recoveries (> 95%)
Content FISHBright™ Labeling Kits
- PlatinumBright component (fluorochromes or haptens)
- 10 x Labeling Solution
- KREApure purification columns
- Hybridization buffer for cytological preps (CHB)
- Hybridization buffer for paraffin preps (THB KREAboost)
The FISHBright™ Kit contains sufficient solution to label up to 10 μg of BAC DNA, which generally allows to perform 100 or more FISH assays using 10 μl labeled probe in hybridization buffer (amount of FISH assays is dependent on the final concentration of labeled DNA. Usually 50 – 100 ng/ μl of labeled BAC DNA is necessary per assay).
FISHgrade C0t
FISHgrade C0t DNA is used to suppress cross-hybridization to human repetitive DNA in FISH experiments. FISHgrade C0t is a high quality C0t-1 DNA that effectively represses repetitive sequences in in situ hybridization experiments. It works perfectly for probes labeled with FISHBright ULS-Kits.