Until recently, the potential drawback of a CISH assay has been the fact that most such assays are based on single-color thereby preventing the possibility to study ratio of genes, like for the Her-2 gene in relation to the centromeric region of chromosome 17. TwinStar provides a universal solution capable of transforming all of Kreatech´s dual-color FISH probes into chromogenic signals. Three complete kits are available designed for detecting Her-2/Neu, EGFR, and the membrane bound receptor C-MET, respectively. Each of these include the corresponding FISH probes and the specific TwinStar detection module converting both signals into chromogenic signals via a colorimetric assay system.
The TwinStar CISH Detection Kit is a module of all TwinStar assays including all reagents required to perform dual-color CISH. It therefore can also be ordered stand-alone and possibly be combined with other POSEIDON FISH probes.
Technical use
TwinStar is designed for dual-color CISH. The detection part includes two proprietary substrates converting both fluorescent signals into distinct colorimetric signals, the red fluor is converted into a red signal and the green fluor into a grayish-green signal. In addition to the complete kits listed above, a dual-color CISH assay can therefore be employed for all of our solid tumor probes as long as these are labeled with our red and green fluorophore.
Amplified Her-2/Neu (ERBB2) on a breast cancer specimen visualized with the TwinStar dual color CISH kit.
Until recently, the potential drawback of a CISH assay has been the fact that most such assays are based on single-color thereby preventing the possibility to study ratio of genes, like for the Her-2 gene in relation to the centromeric region of chromosome 17. TwinStar provides a universal solution capable of transforming all of Kreatech´s dual-color FISH probes into chromogenic signals. Three complete kits are available designed for detecting Her-2/Neu, EGFR, and the membrane bound receptor C-MET, respectively. Each of these include the corresponding FISH probes and the specific TwinStar detection module converting both signals into chromogenic signals via a colorimetric assay system.
The TwinStar CISH Detection Kit is a module of all TwinStar assays including all reagents required to perform dual-color CISH. It therefore can also be ordered stand-alone and possibly be combined with other POSEIDON FISH probes.
Technical use
TwinStar is designed for dual-color CISH. The detection part includes two proprietary substrates converting both fluorescent signals into distinct colorimetric signals, the red fluor is converted into a red signal and the green fluor into a grayish-green signal. In addition to the complete kits listed above, a dual-color CISH assay can therefore be employed for all of our solid tumor probes as long as these are labeled with our red and green fluorophore.
Amplified Her-2/Neu (ERBB2) on a breast cancer specimen visualized with the TwinStar dual color CISH kit.