Introduction
FISH is the current gold standard to determine the chromosomal constitution of an embryo. In contrast to karyotyping, it can be used on interphase chromosomes, so that it can be applied on polar bodies, blastomeres and other single cell samples. FISH is therefore accepted as a routine method in preimplantation genetic screening (PGS) in determining chromosome aneuploidies prior to implanting an embyro and increases the success rate of an IVF-mediated pregnancy.
Currently, a large panel of FISH probes are available for different segments of all chromosomes, but until recently the limited number of different fluorochromes has confined the number of signals that can be analyzed simultaneously.
Kreatech is pleased to introduce a unique multicolor FISH panel enabling visualization of all 24 chromosomes in a single cell. This novel method thereby eliminates the bottleneck perceived when using FISH by omitting key chromosomes relevant to implantation failure not covered in a limited panel. Relatively few screened chromosomes
may be sufficient to identify non-viable abnormalities in women over 40, but younger women or those with repeated IVF failure may benefit from comprehensive 24 chromosome screening.
Introduction
FISH is the current gold standard to determine the chromosomal constitution of an embryo. In contrast to karyotyping, it can be used on interphase chromosomes, so that it can be applied on polar bodies, blastomeres and other single cell samples. FISH is therefore accepted as a routine method in preimplantation genetic screening (PGS) in determining chromosome aneuploidies prior to implanting an embyro and increases the success rate of an IVF-mediated pregnancy.
Currently, a large panel of FISH probes are available for different segments of all chromosomes, but until recently the limited number of different fluorochromes has confined the number of signals that can be analyzed simultaneously.
Kreatech is pleased to introduce a unique multicolor FISH panel enabling visualization of all 24 chromosomes in a single cell. This novel method thereby eliminates the bottleneck perceived when using FISH by omitting key chromosomes relevant to implantation failure not covered in a limited panel. Relatively few screened chromosomes
may be sufficient to identify non-viable abnormalities in women over 40, but younger women or those with repeated IVF failure may benefit from comprehensive 24 chromosome screening.