The ULS arrayCGH Labeling Kit offers a novel procedure that allows direct (non-enzymatic) labeling of both intact genomic DNA as well as fragmented genomic DNA from formalin-fixed paraffin-embedded (FFPE) samples. The ULS arrayCGH Labeling Kit yields highly reproducible fluorescent labeled DNA within minutes. The ULS technology has been validated on BAC array CGH platforms. All ULS kits for array CGH analysis include the KREApure purification technology and KREAblock blocking reagents to ensure efficient purification of labeled DNA samples and low background levels during hybridization.
Unique features of ULS arrayCGH Labeling Kit
- 30 min labeling procedure
- Labels fragmented genomic DNA isolated from FFPE samples equally well than undegraded DNA samples
- ULS labeling is not affected by cross links present in genomic DNA from FFPE samples
- No enzymatic bias
- KREApure column purification ideally suited for fragmented DNA.
Time of ULS labeling vs. competitive labeling procedure
ULS Labeling vs Random Prime Labeling on BAC arrays
Ratio plots of 3 independent array CGH hybridizations using genomic DNA from healthy liver which is the reference vs. genomic DNA isolated from a liver tumor FFPE sample. (A-C) ULS labeled samples. (D-F) Random Prime labeled samples.
The ULS arrayCGH Labeling Kit offers a novel procedure that allows direct (non-enzymatic) labeling of both intact genomic DNA as well as fragmented genomic DNA from formalin-fixed paraffin-embedded (FFPE) samples. The ULS arrayCGH Labeling Kit yields highly reproducible fluorescent labeled DNA within minutes. The ULS technology has been validated on BAC array CGH platforms. All ULS kits for array CGH analysis include the KREApure purification technology and KREAblock blocking reagents to ensure efficient purification of labeled DNA samples and low background levels during hybridization.
Unique features of ULS arrayCGH Labeling Kit
- 30 min labeling procedure
- Labels fragmented genomic DNA isolated from FFPE samples equally well than undegraded DNA samples
- ULS labeling is not affected by cross links present in genomic DNA from FFPE samples
- No enzymatic bias
- KREApure column purification ideally suited for fragmented DNA.
Time of ULS labeling vs. competitive labeling procedure
ULS Labeling vs Random Prime Labeling on BAC arrays
Ratio plots of 3 independent array CGH hybridizations using genomic DNA from healthy liver which is the reference vs. genomic DNA isolated from a liver tumor FFPE sample. (A-C) ULS labeled samples. (D-F) Random Prime labeled samples.