The PlatinumLink Kits provide high coverage of the proteome through specific labeling of methionine, cysteine
and histidine residues. Unlike lysine, which is targeted by N-hydroxysuccinimidyl (NHS) esters, the amino acids labeled with ULS are less likely to be involved in protein - protein interactions. Therefore, labeling of proteins and antibodies with ULS will reduce negative effects on interaction domains and epitope recognition.
The PlatinumLink Antibody Labeling Kits have been optimized to label 100 μg of purified antibody (or recombinant protein) per reaction with fluorescent dyes or haptens.
Unique features of the PlatinumLink Antibody Labeling Kits
- Easy and robust labeling of antibodies and recombinant proteins
- ULS-Trap removes unreacted label, no size exclusion
- Labeling of methionines, cysteines and histidines in antibodies and recombinant proteins
- Labeling reaction is independent of pH
- Compatible with commonly used detergents including Tris-HCl.
Schematic overview of the PlatinumLink labeling procedure.
Recovery comparison of different proteins.
Protein recovery using ULS-Trap columns 100 μg of protein was labeled and purified using PlatinumLink protein labeling kits. Protein concentration was determined using Biorad’s Rc Dc protein assay. Recovery is presented as percentage protein recovered as compared to unpurified sample.
The PlatinumLink Kits provide high coverage of the proteome through specific labeling of methionine, cysteine
and histidine residues. Unlike lysine, which is targeted by N-hydroxysuccinimidyl (NHS) esters, the amino acids labeled with ULS are less likely to be involved in protein - protein interactions. Therefore, labeling of proteins and antibodies with ULS will reduce negative effects on interaction domains and epitope recognition.
The PlatinumLink Antibody Labeling Kits have been optimized to label 100 μg of purified antibody (or recombinant protein) per reaction with fluorescent dyes or haptens.
Unique features of the PlatinumLink Antibody Labeling Kits
- Easy and robust labeling of antibodies and recombinant proteins
- ULS-Trap removes unreacted label, no size exclusion
- Labeling of methionines, cysteines and histidines in antibodies and recombinant proteins
- Labeling reaction is independent of pH
- Compatible with commonly used detergents including Tris-HCl.
Schematic overview of the PlatinumLink labeling procedure.
Recovery comparison of different proteins.
Protein recovery using ULS-Trap columns 100 μg of protein was labeled and purified using PlatinumLink protein labeling kits. Protein concentration was determined using Biorad’s Rc Dc protein assay. Recovery is presented as percentage protein recovered as compared to unpurified sample.