Histostain®-SP (Streptavidin-Peroxidase) and Histostain®-SAP (Streptavidin-Alkaline Phosphatase) immunohistochemical staining kits employ the LAB-SA (Labeled [strept]Avidin-Biotin) method. For Histostain®-SP Kits, DAB or AEC chromogen/substrate generates a colored precipitate around the target antigen in human samples. DAB (brown) is approximately twice as sensitive as AEC (reddish). For Histostain®-SAP Kits, AP-Red or BCIP/NBT chromogen/substrate generates a colored precipitate around the target antigen in human samples. BCIP/NBT (dark blue/purple) is about twice as sensitive as AP-Red (red/magenta).
All components are liquid and come in easy-to-use, color-coded dropper bottles. Most reagents are supplied in prediluted form at working strength; substrate reagents require a simple dilution. No ABC complex formation, no reconstitution, and no pipetting are required. Ask us about using these kits with nonhuman samples.
Rabbit anti-synaptophysin stained islet cell tumor using a Histostain®-SAP Broad Spectrum Kit (AP-Red) counterstained with hematoxylin.
Mouse anti-cytokeratin stained skin squamous carcinoma using a Histostain®-SP Broad Spectrum kit (DAB) and hematoxylin counterstain.
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Advantages:
- Exceptionally clean staining
- Outstanding value
- Flexible kits are well suited to custom protocols
- Color-coded solutions
- Fast (10/10 minute incubations for 2° Ab and enzyme conjugate)
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Kit contents:
1A Blocking Solution (ready-to-use)
1B Biotinylated Secondary Antibody (ready-to-use)
2 Streptavidin-HRP for Histostain®-SP Kits (ready-to-use) OR Streptavidin-AP for Histostain®-SAP Kits (ready-to-use) 3A Substrate Buffer † (20x)
3B Chromogen Solution † (20x)
3C Hydrogen Peroxide † (20x)
4 Hematoxylin Solution † (ready-to-use)
5 Mounting Solution † (ready-to-use)
† Bulk kit does not contain Chromogen/Substrate.
Histostain®-SP (Streptavidin-Peroxidase) and Histostain®-SAP (Streptavidin-Alkaline Phosphatase) immunohistochemical staining kits employ the LAB-SA (Labeled [strept]Avidin-Biotin) method. For Histostain®-SP Kits, DAB or AEC chromogen/substrate generates a colored precipitate around the target antigen in human samples. DAB (brown) is approximately twice as sensitive as AEC (reddish). For Histostain®-SAP Kits, AP-Red or BCIP/NBT chromogen/substrate generates a colored precipitate around the target antigen in human samples. BCIP/NBT (dark blue/purple) is about twice as sensitive as AP-Red (red/magenta).
All components are liquid and come in easy-to-use, color-coded dropper bottles. Most reagents are supplied in prediluted form at working strength; substrate reagents require a simple dilution. No ABC complex formation, no reconstitution, and no pipetting are required. Ask us about using these kits with nonhuman samples.
Rabbit anti-synaptophysin stained islet cell tumor using a Histostain®-SAP Broad Spectrum Kit (AP-Red) counterstained with hematoxylin.
Mouse anti-cytokeratin stained skin squamous carcinoma using a Histostain®-SP Broad Spectrum kit (DAB) and hematoxylin counterstain.
|
Advantages:
- Exceptionally clean staining
- Outstanding value
- Flexible kits are well suited to custom protocols
- Color-coded solutions
- Fast (10/10 minute incubations for 2° Ab and enzyme conjugate)
|
Kit contents:
1A Blocking Solution (ready-to-use)
1B Biotinylated Secondary Antibody (ready-to-use)
2 Streptavidin-HRP for Histostain®-SP Kits (ready-to-use) OR Streptavidin-AP for Histostain®-SAP Kits (ready-to-use) 3A Substrate Buffer † (20x)
3B Chromogen Solution † (20x)
3C Hydrogen Peroxide † (20x)
4 Hematoxylin Solution † (ready-to-use)
5 Mounting Solution † (ready-to-use)
† Bulk kit does not contain Chromogen/Substrate.