Introduction
The primary constriction, called the centromer, is a common feature of chromosomes necessary for cell division. Presence of repetitive sequences in the centromeric regions have been proven to be essential. In humans, and many other species, specific repetitive sequences, called ‘Satellites’ are characteristic for the centromer in general. Most chromosomes have even repetitive sequences which are specific for individual chromosomes and can be used for precise identification and enumeration of human chromosomes in metaphase and interphase cells.
The Poseidon Satellite Enumeration probes – SE – allow rapid and specific chromosome analysis, marker chromosome identification and the detection of aneuploidy. Essentially, these probes can be used in all aspects of routine work in genetics and oncology/pathology. Due to the sharp and bright signals produced, the Satellite probes can easily be used on various sample types, such as cultured cells, touch preparations buccal smears, cytospins, frozen and paraffin-embedded tissue sections, sputum samples, sperm samples, and bladder washes.
Technical Information
All SE probes are supplied in a 10 test format together with hybridization buffer, labeled in a choice of green (PlatinumBright 495) or red (PlatinumBright 550). All SE probes can also be supplied labeled in blue (PlatinumBright 415) on request.
The SE combinations are already supplied in a Dual-Color format ready-to-use. Poseidon SE probes are provided in concentrated format to allow, if required, mixing of up to 5 SE probes in a single hybridization assay. Please refer to the Instruction for Use Manual for further details.
Note:
Satellite sequences share some degree of homology between the sequences from chromosome to chromosome. Therefore the recommended stringency Conditions in hybridization and posthybridization washes must be followed to provide optimal results. Chromosomes 1/5/19, 13/21, and 14/22 share the same repetitive sequences and cannot be differentiated by chromosome specific repeats.
Introduction
The primary constriction, called the centromer, is a common feature of chromosomes necessary for cell division. Presence of repetitive sequences in the centromeric regions have been proven to be essential. In humans, and many other species, specific repetitive sequences, called ‘Satellites’ are characteristic for the centromer in general. Most chromosomes have even repetitive sequences which are specific for individual chromosomes and can be used for precise identification and enumeration of human chromosomes in metaphase and interphase cells.
The Poseidon Satellite Enumeration probes – SE – allow rapid and specific chromosome analysis, marker chromosome identification and the detection of aneuploidy. Essentially, these probes can be used in all aspects of routine work in genetics and oncology/pathology. Due to the sharp and bright signals produced, the Satellite probes can easily be used on various sample types, such as cultured cells, touch preparations buccal smears, cytospins, frozen and paraffin-embedded tissue sections, sputum samples, sperm samples, and bladder washes.
Technical Information
All SE probes are supplied in a 10 test format together with hybridization buffer, labeled in a choice of green (PlatinumBright 495) or red (PlatinumBright 550). All SE probes can also be supplied labeled in blue (PlatinumBright 415) on request.
The SE combinations are already supplied in a Dual-Color format ready-to-use. Poseidon SE probes are provided in concentrated format to allow, if required, mixing of up to 5 SE probes in a single hybridization assay. Please refer to the Instruction for Use Manual for further details.
Note:
Satellite sequences share some degree of homology between the sequences from chromosome to chromosome. Therefore the recommended stringency Conditions in hybridization and posthybridization washes must be followed to provide optimal results. Chromosomes 1/5/19, 13/21, and 14/22 share the same repetitive sequences and cannot be differentiated by chromosome specific repeats.