Introduction
The Poseidon Whole Chromosome probes hybridize to unique sequences spanning the entire length of the target chromosome.
These probes are derived from flow-sorted or microdissected chromosomes and provide accurate coverage with excellent signal specificity and high fluorescent intensity. Some minor cross-hybridization may occur at the short arm of acrocentric chromosomes (13, 14, 15, 21 and 22) and in the pseudo-autosomal regions of chromosome X and Y.
The WC probes are used for identifying whole human chromosomes, analysis of translocation events, chromosome rearrangement studies and determining the origin of marker chromosomes. Mutagenesis analysis, radiation and sensitivity testing, and identification of human chromosomes on hybrid cells are investigated using these probes.
Technical Information
The WC probes are supplied in a 5 test format together with hybridization buffer, labeled in a choice of green (PlatinumBright 495) and red (PlatinumBright 550) or in blue (PlatinumBright 415) (for WC in blue please inquire).
Poseidon WC probes are provided in concentrated format to allow, if required, mixing of up to 5 WC probes in a single hybridization assay. Please refer to the Instruction for Use Manual for further details.
Note:
Due to decondensation of chromosomal DNA signals on interphase, cells can be very diffuse. We therefore recommend not to use WC probes for interphase FISH.
Introduction
The Poseidon Whole Chromosome probes hybridize to unique sequences spanning the entire length of the target chromosome.
These probes are derived from flow-sorted or microdissected chromosomes and provide accurate coverage with excellent signal specificity and high fluorescent intensity. Some minor cross-hybridization may occur at the short arm of acrocentric chromosomes (13, 14, 15, 21 and 22) and in the pseudo-autosomal regions of chromosome X and Y.
The WC probes are used for identifying whole human chromosomes, analysis of translocation events, chromosome rearrangement studies and determining the origin of marker chromosomes. Mutagenesis analysis, radiation and sensitivity testing, and identification of human chromosomes on hybrid cells are investigated using these probes.
Technical Information
The WC probes are supplied in a 5 test format together with hybridization buffer, labeled in a choice of green (PlatinumBright 495) and red (PlatinumBright 550) or in blue (PlatinumBright 415) (for WC in blue please inquire).
Poseidon WC probes are provided in concentrated format to allow, if required, mixing of up to 5 WC probes in a single hybridization assay. Please refer to the Instruction for Use Manual for further details.
Note:
Due to decondensation of chromosomal DNA signals on interphase, cells can be very diffuse. We therefore recommend not to use WC probes for interphase FISH.