REPEAT-FREE™ FISH Technology

REPEAT-FREE™ POSEIDON™ FISH procedure VS. Conventional FISH procedure
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REPEAT-FREE™ (RF) POSEIDON™ FISH PROBES are the latest advancements in DNA FISH probes. The RF technology used to construct the RF POSEIDON™ FISH probes is based on subtractive hybridization that specifically removes all repetitive elements that are dispersed throughout the target area of interest. Eliminating these repeat sequences leads to more specific binding kinetics and eliminates the need to use Cot1 DNA during pre-annealing. This results in FISH assays with reduced backgrounds and brighter signals. Traditional FISH is time consuming with standard hybridizations being 16 hours on average. With the use of RF POSEIDON™ probes, hybridization may be shortened to as little as 4 hours depending on the combination of probe and sample type used. RF technology can have a positive impact on the readability, sensitivity and specificity of your FISH assays.

REPEAT-FREE™ POSEIDON™ FISH procedure VS. Conventional FISH procedure
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In addition, our DNA probes are labeled with Kreatech’s proprietary ULS™ linkage system leading to a very homogenous and consistent labeling of fluorescent dyes.

The signal to noise ratio of RF POSEIDON FISH probes in comparison to standard probes today is 1.5 to 3 times greater.

Our recommended Pretreatment Kits with Ready-to-Use reagents makes the pretreatment of different tissue and cytological samples easier and guarantees optimal results in combination with the RF POSEIDON FISH probes.

Human Chromosome Poster

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Mouse Chromosome Poster

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